(Methods in Molecular Biology (937)) Softcover reprint of the original 3rd ed. 2013 Edition
by David G. Lambert (Editor), Richard D. Rainbow (Editor)
The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols,
and the developments in the field have also, naturally, continued.
With the third edition, expert contributors explore some of the exciting
new molecular techniques that have both enabled new studies of
intracellular Ca2+ regulation and provided much new
information on processes. Comprised of five main section, the book
covers theoretical and very simple suspension-based fluorimetric assays,
specialist measurement systems, measurement of channel activity,
measurement of store release, as well as specialist measurement
techniques which include targeted probes, using G-protein chimeras to
force Ca2+ signalling for screening, and genetically encoded sensors. Written in the highly successful Methods in Molecular Biology™
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting
and avoiding known pitfalls.
Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca2+.
