(Springer Protocols Handbooks) 1st ed. 2020 Edition
by Monalisa Mishra (Editor)
This volume includes in-depth, hands-on protocols for detecting
various developmental defects in Drosophila. It provides cutting-edge
methods for maintaining Drosophila under laboratory conditions to
perform various experiments, and for dissecting various imaginal discs
of the larvae. Further, biochemical protocols for estimating the levels
of different metabolites, reactive oxygen species, and fat-sensitive
pathways are discussed, and various staining and behavior techniques for
fat detection are provided.
The book explains how various
fluorescent dyes and the comet assay can be used to identify DNA damage,
and elaborates on the analysis of the eye, antennae, imaginal disc, gut
and muscle damage under bright field and fluorescent microscopes. It
covers the analysis of hemolymph using Giemsa staining; determining the
functionality of channel proteins, eye and mechanosensory organs in
adults and larvae using various behavioral assays; and metal detection
and structural analysis for various tissues using SEM. In closing, it
addresses the analysis of Drosophila’s eye and head via paraffin
section; measurement of reactive oxygen species from various tissues via
FACS; and the CRISPER technique for gene editing and analysis of micro
RNA mutations.
