(Methods in Pharmacology and Toxicology) 2014th Edition
by L. María Sierra (Editor), Isabel Gaivão (Editor)
Genotoxicity and DNA Repair: A Practical Approach
provides a key reference for determining how to analyze the genotoxic
activity of molecules or materials and, at the same time, serves as a
useful tool for researchers in the Environmental Mutagenesis and DNA
Repair fields. Focused on genotoxicity assays recommended by the “OECD
guidelines for the testing of chemicals”, this volume also covers other
useful assays, such as some gene mutation assays, the comet assay in
different species and applications, and the SMART assays of Drosophila.
For all the assays, the book presents brief theoretical introductions to
the topics and updated standard and modified step-by-step protocols to
perform them. Special emphasis is placed on the analysis of
nanoparticles, including an integrative approach analysis. The DNA
Repair section includes several assays that provide information on
repair activity in vitro and in vivo, as well as recent applications to
study DNA repair in humans, cell cultures, and animal models. As a
volume in the Methods in Pharmacology and Toxicology series, the chapters contain the kind of detail and key implementation advice that ensures reproducible results in the lab.
Authoritative and invaluable, Genotoxicity and DNA Repair: A Practical Approach aims to aid scientists in their pursuit of forwarding this vital field of study.
