2nd Edition
by Michael W. Dong (Author)
A concise yet comprehensive
reference guide on HPLC/UHPLC that focuses on its fundamentals, latest
developments, and best practices in the pharmaceutical and biotechnology
industries
Written for practitioners by an expert practitioner, this new edition of HPLC and UHPLC for Practicing Scientists
adds numerous updates to its coverage of high-performance liquid
chromatography, including comprehensive information on UHPLC
(ultra-high-pressure liquid chromatography) and the continuing migration
of HPLC to UHPLC, the modern standard platform. In addition to
introducing readers to HPLC’s fundamentals, applications, and
developments, the book describes basic theory and terminology for the
novice, and reviews relevant concepts, best practices, and modern trends
for the experienced practitioner.
HPLC and UHPLC for Practicing Scientists, Second Edition
offers three new chapters. One is a standalone chapter on UHPLC,
covering concepts, benefits, practices, and potential issues. Another
examines liquid chromatography/mass spectrometry (LC/MS). The third
reviews at the analysis of recombinant biologics, particularly
monoclonal antibodies (mAbs), used as therapeutics. While all chapters
are revised in the new edition, five chapters are essentially rewritten
(HPLC columns, instrumentation, pharmaceutical analysis, method
development, and regulatory aspects). The book also includes problem and
answer sections at the end of each chapter.
- Overviews
fundamentals of HPLC to UHPLC, including theories, columns, and
instruments with an abundance of tables, figures, and key references
- Features brand new chapters on UHPLC, LC/MS, and analysis of recombinant biologics
- Presents
updated information on the best practices in method development,
validation, operation, troubleshooting, and maintaining regulatory
compliance for both HPLC and UHPLC
- Contains major revisions to
all chapters of the first edition and substantial rewrites of chapters
on HPLC columns, instrumentation, pharmaceutical analysis, method
development, and regulatory aspects
- Includes end-of-chapter quizzes as assessment and learning aids
- Offers a reference guide to graduate students and practicing scientists in pharmaceutical, biotechnology, and other industries
Filled with intuitive explanations, case studies, and clear figures, HPLC and UHPLC for Practicing Scientists, Second Edition
is an essential resource for practitioners of all levels who need to
understand and utilize this versatile analytical technology. It will be a
great benefit to every busy laboratory analyst and researcher.
