(Methods in Molecular Biology) 1st ed. 2019 Edition
by Petros Papagerakis (Editor)
This volume provides methods and approaches to study genetic and
environmental regulatory controls on odontogenesis. Chapters guide
readers through protocols for isolation and characterization of both
epithelial and mesenchymal dental cells, methods on isolation,
phenotypic characterization, expansion, differentiation,
immunofluorescence, in situ hybridization, immunohistochemistry, imaging
protocols, rodent dental fluorosis model, 3D assessment of crown size,
dental diseases models, next generation sequencing, genetic and
epigenetic studies, genome-wide association studies as well as clinical
protocols for measurement of early childhood caries and saliva, and
supragingival fluids and biofilm collection and subsequent analyses.
Written in the highly successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Authoritative and cutting-edge, Odontogenesis: Methods and Protocols aims to guide researchers towards elucidating the secrets and mysteries of a fascinating and unique organ, the tooth.
