English | 2023 | PDF | 18 MB | 228 Pages
Suresh Kumar Subbiah, Akon Higuchi; Pooi Ling Mok, 9780128237298, 978-0128237298, 978-0-12-823729-8, B0C6HX3MJH, 0128237295, 0128237309, 9780128237304, 978-0128237304
Stem Cell Laboratory Techniques: A Guide
for Researchers and Students introduces the reader to stem cell
culture, handling techniques and versatile applications used by
researchers. Sections introduce stem cells, including definitions, types
and basic use of stem cells in biomedical science research. The book
explains laboratory procedures and techniques ranging from the
extraction of stem cells from animals, cell seeding and culture, harvest
and maintenance of stem cells, stem cell characterization, accurate
recording, quality control, and more. In addition, it guides researchers
on topics such as transcriptome analysis, proliferation study analysis,
and microphysiological study.
Final sections cover useful and recent
applications in stem cells, such as gene editing techniques and the
preparation of stem cells for in vitro study, as well as stem cell lab
design and equipment used in the lab. Lastly, human and animal research
ethics are discussed.
- Introduces readers to the stem cell culture and moves to handling techniques and versatile applications
- Includes coverage of gene editing techniques for stem cells and stem cells for in vitro study
- Presents stem cell lab design and equipment used in the lab
