(Methods in Molecular Biology (1017)) 2013th Edition
by Danny M. Hatters (Editor), Anthony J. Hannan (Editor)
The genomes of humans, as well as
many other species, are interspersed with hundreds of thousands of
tandem repeats of DNA sequences. Those tandem repeats located as codons
within open reading frames encode amino acid runs, such as
polyglutamine and polyalanine. Tandem repeats have not only been
implicated in biological evolution, development and function but also in
a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert
researchers in the field detail many methods covering the analysis of
tandem repeats in DNA, RNA and protein, in healthy and diseased states.
This will include molecular genetics, molecular biology, biochemistry,
proteomics, biophysics, cell biology, and molecular and cellular
approaches to animal models of tandem repeat disorders. Written in the
highly successful Methods in Molecular Biology™ series format,
chapters include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible
laboratory protocols, and key tips on troubleshooting and avoiding known
pitfalls.
Authoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols
aids scientists in continuing to study the unique methodological
challenges that come from repetitive DNA and poly-amino acid sequences.
