by Wendy A. Harwood
This
detailed volume explores barley as both a crop and a model, with
practical techniques such as crossing barley, a range of tissue culture
methods, the preparation of barley tissues for different forms of
microscopy, and the assessment of sensitivity to abiotic stresses.
Efficient protocols are provided for transformation, TILLING,
virus-induced gene silencing and genome editing. There is also
particular emphasis on a range of protocols for genotyping and for the
analysis of gene expression. Written for the highly successful Methods
in Molecular Biology series, chapters include introductions on their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.
Authoritative
and easy-to-use, Barley: Methods and Protocols serves as a valuable
reference volume for cereal researchers and breeders by providing
detailed protocols covering important traditional skills such as
crossing and tissue culture through to the latest technologies for
genotyping, expression analysis, and genome editing.
