(Methods in Molecular Biology) 2015th Edition
by Waltraud X. Schulze (Editor)
This detailed volume addresses
recent developments in phosphoproteomic techniques with a particular
focus on the plant system. Over the recent decades, proteomic methods
were refined to study the significance and dynamics of protein
phosphorylation in various biological contexts. However, working with
plant tissue imposes particular challenges to the biologist which are
attributed to the rigid cell wall making protein extraction more
difficult, the skewed protein abundance with Rubisco as a highly
abundant protein and a large central vacuole leading to low protein
yield and increased degradative enzyme activity. The methodologies in
this book seek to move beyond these issues. Written for the Methods in Molecular Biology
series, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols and tips on troubleshooting and
avoiding known pitfalls.
Practical and authoritative, Plant Phosphoproteomics: Methods and Protocols serves as an ideal reference for researchers investigating this vital area of plant science.
