4th Edition
by Roger L. Lundblad (Author)
The use of the chemical
modification of proteins has evolved over the past 80 years, benefiting
from advances in analytical, physical, and organic chemistry. Over the
past 30 years, the use of chemical reagents to modify proteins has been
crucial in determining the function and structure of purified proteins.
This groundbreaking work is part of the foundation of emerging
disciplines of proteomics, chemical biology, structure biology, and
chemical proteomics.
Chemical Reagents for Protein Modification, Fourth Edition
provides a comprehensive review of reagents used for the chemical
modification of proteins, representing a major revision of the work
presented in previous editions. The completely updated Fourth Edition is
substantially larger and includes five new chapters:
- Alkylating Agents
- Acylating Agents
- Nitration and Nitrosylation
- Oxidation
- Modification of Proteins with Reducing Agents
There
is greatly increased coverage of the chemical modification of cysteine,
which is critical for bioconjugate synthesis. The chapter on reduction
also provides information necessary for bioconjugate synthesis as well
as for the processing of inclusion bodies. The book places emphasis on
conditions that affect the specificity of the chemical modification of
proteins, such as solvent and temperature. The format has been markedly
revised, presenting information based on the chemical nature of the
modifying material and on the amino acid residue modified. This new
version has increased significance to biopharmaceuticals. Much of the
information is in tabular form, which enables the rapid location of
cited material.